"Biophysics-driven Multi-lineage Induction in Engineered Cardiac Organoids"
Zhen Ma, Ph.D.
Carol and Samuel Nappi Research Scholar
Department of Biomedical & Chemical Engineering
Syracuse Biomaterials Institute
Syracuse Soft & Living Matters
Human induced pluripotent stem cells (hiPSCs) have altered the landscape of regenerative medicine and developmental biology, since these cells provide an unprecedented opportunity to study human-specific tissue morphogenesis and organ development. With the emergent concept of stem cell organoids, these 3D cultures of developing tissue imply similarity to the manner in which different organs establish their characteristic structure and functions based on dynamic multicellular self-organization at the tissue level. My research group has developed a spatial-organized 3D organoid model that captures the dynamic process of early human organ development during embryogenesis. By varying the geometric shape of patterned hiPSCs, we have achieved multi-lineage induction with co-emergent cell specification of cardiac lineage, stromal lineage and hepatic lineage. Our multi-lineage organoids not only recaptured the parallel organ development of the heart-liver synergy, but also demonstrated that biophysical cues can modulate the co-emergent developmental process by shifting cell lineages between mesoderm and endoderm. More recently, to elucidate structure-function relationship for cardiac organoids, we applied advanced data mining techniques for analysis and visualization of sophisticated, large-scale multidimensional “Physiomics” datasets from these organoids.
The Bioengineering Seminar Series is co-hosted by the Parker H. Petit Institute for Bioengineering and Bioscience, and the Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University.